The Tyler Medical Clinic

Assisted Conception - Affordable Infertility Treatments


Home FAQ Press Glossary Contact Us

About Tyler Medical Procedures Endometriosis Institute Westwood Cryobank IRMGT

Sperm Freezing
Testicular Tissue Freezing
Egg Freezing
Ovarian Tissue Freezing

- Testicular Tissue Cryopreservation -

 

Obtaining the tissue sample

Testicular biopsy is performed.  Retrieved tissue is cut into small pieces.  

Separating the sperm

Seminiferous tubules are isolated and incubated in collagenase for 1 hour.  The tubules are then rinsed in fresh medium and stripped of sperm using needles and fine tweezers. 

Cryopreservation

Tissue and sperm are mixed with cryoprotectant solution (glycerol) and deposited in cryo-vials.  The cryo-vials are placed into liquid nitrogen vapor for 3 hours and then plunged into liquid nitrogen.  The sample is preserved in liquid nitrogen at temperatures of -196°C until needed IUI/IVF/ICSI.

 

Up


Back Home Up Next

The contents of The Tyler Medical Clinic Site, such as text, graphics, images, and other material ("Content") are for informational purposes only. The Content is not intended to be a substitute for professional medical advice, diagnosis, or treatment. Always seek the advice of your physician or other qualified health provider with any questions you may have regarding a medical condition. Never disregard professional medical advice or delay in seeking it because of something you have read on The Tyler Medical Clinic Site!

Copyright © 2001 The Tyler Medical Clinic
Last modified: 06/09/04