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The Tyler Medical Clinic Assisted Conception - Affordable Infertility Treatments
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- Testicular Tissue Cryopreservation -
Obtaining the tissue sample Testicular biopsy is performed. Retrieved tissue is cut into small pieces. Separating the sperm Seminiferous tubules are isolated and incubated in collagenase for 1 hour. The tubules are then rinsed in fresh medium and stripped of sperm using needles and fine tweezers. Cryopreservation Tissue and sperm are mixed with cryoprotectant solution (glycerol) and deposited in cryo-vials. The cryo-vials are placed into liquid nitrogen vapor for 3 hours and then plunged into liquid nitrogen. The sample is preserved in liquid nitrogen at temperatures of -196°C until needed IUI/IVF/ICSI.
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The Tyler Medical Clinic
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