The Tyler Medical Clinic
Assisted Conception - Affordable Infertility Treatments
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The Tyler Medical Clinic Assisted Conception - Affordable Infertility Treatments
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- Ovarian Tissue Cryopreservation -
Ovarian tissue have been found to survive well using improved cryoprotectant solutions and techniques. These new developments offer the hope of preserving fertility for a large number of patients. Ovarian Tissue Retrieval Ovarian tissue is removed through a laparoscopic procedure. A portion of the ovary is sectioned into thin tissue slices. Treatment with cryoprotectant Ovarian tissue must be treated with a cryoprotectant before they can be frozen. A cryoprotectant such as ethylene glycol (EG) is added to a physiological saline in 0.5 M increments every 5 minutes until the desired concentration (1.5 M EG) is reached. The sample, processed at ambient temperature, is now ready for freezing. Ovarian tissue freezing and storage The temperature of the sample is reduced from 20° to -7°C (the temperature of ice nucleation) at 2°C/minute, then held for 10 minutes. Cooling is then resumed at a rate of -0.5°C/minute to -30°C. The frozen tissue is then plunged into liquid nitrogen for storage.
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The Tyler Medical Clinic
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